Can we add salicylic acid in culture media prior to autoclaving or we should first filter sterilize SA and than it should be added to the media?
I have read that the melting point of SA is 158.6 °C whereas the boiling point is 211 °C.
According to this book (Handbook of Microbiological Media, Fourth Edition; see below link), it should be filter sterilized and then add to autoclaved medium.
https://books.google.com/books?id=NpbLBQAAQBAJ&pg=PA1182&lpg=PA1182&dq=how+to+sterilize+salicylic+acid+for+medium&source=bl&ots=QAi4wZ_pCA&sig=2zm0s__jMRYIGGv77ADQVivjmlE&hl=en&sa=X&ved=0ahUKEwi9_u2L-qnZAhXjq1QKHU0FDXwQ6AEIQDAE#v=onepage&q=how%20to%20sterilize%20salicylic%20acid%20for%20medium&f=false
Thank you sir
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