we work on skin fibroblasts and for flow analysis we use CD140a (PDGFRa; clone APA5) in combination with CD45 (here as an exclusion marker). Also on histological skin sections of PDGFRa-GFP mice we see very nicely that CD140a is a very good marker for this cell type. Still, make sure to include CD45 in flow to be even more specific (in addition to FSC/SSC properties of the cells).
we work on skin fibroblasts and for flow analysis we use CD140a (PDGFRa; clone APA5) in combination with CD45 (here as an exclusion marker). Also on histological skin sections of PDGFRa-GFP mice we see very nicely that CD140a is a very good marker for this cell type. Still, make sure to include CD45 in flow to be even more specific (in addition to FSC/SSC properties of the cells).