In a batch bioreactor operation, sampling amount of 1 ml and equal amount addition makes this process a fed-batch?
In a batch bioreactor operation, adding an equal amount of 1 ml sample does not make the process a fed-batch. In a fed-batch culture, nutrients are added to the bioreactor during cultivation, and the volume of liquid culture in the bioreactor increases as the culture is systematically added. Fed-batch culture is a modified version of batch fermentation that allows for long-term synthesis of products, increases yield and productivity with controlled sequential additions of nutrients, enables higher cell densities, and prolongs product synthesis. In contrast, batch fermentation is a process in which all nutrients are provided at the beginning of the cultivation without adding any more in the subsequent bioprocess. During the entire bioprocess, no additional nutrients are added.
References:
https://microbenotes.com/fed-batch-culture/
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/fed-batch-culture
Hi Haris,
If you prefer, I can offer you a conversation with our scientists who do bioprocess development and GMP manufacture routinely.
Best regards,
Gökhan
Dear Haris Nalakath
Fed- batch fermentation is an upgraded version of batch fermentation.
The process is initiated exactly as a batch fermentation but after a fixed time period you add/FED your bioreactor with controlled amount of nutrients, aseptically, which helps to increase the productivity and cell density. Without harvesting any amount of culture before the end of the process.
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