Dear Endo-community,
I isolated EPCs according to Medina et al. (2010) via collagen and was very happy that it worked. Now I want to broadly characterize them via flowcytometry. There are few markers mentioned in the paper. But I wanted to ask whether experts of you can suggest some markers?
Would be happy for any hints.
http://www.tandfonline.com/doi/full/10.1080/10520295.2016.1250284
Hello Kubrah.
It wouldn't matter that much if you provide defined biomarkers to characterize EPCs or subsequent analyses further. In fact, lots of articles maintaining that this is a true biomarker(s) for separation of EPCs are meaningless, although they are the only option at present.
Cell differentiation is not a static state of cell phenotype, but a dynamic states with ceaseless differentiation that is caused by intrinsic and extrinsic cues all the time.
A single or a few biomarkers, in particular, useful surface makers to all of us never tell everything about the cell phenotypes and prospective cell fates at all.
Regards.
Hello, Kübrah,
you can use CD31, vWF, and eNOS. Or consult followed papers:
1. Aper et al (2013) DOI: 10.1055/s-0032-1315112
2. Theodoridis et al (2015) DOI: 10.1016/j.biomaterials.2015.02.023
Best regards
Developmental Studies Hybridoma Bank at the University of Iowa has a plethora of antibodies to endothelial cells. Just check their catalog for ones you may want to use.
In addition to mentioned above comments, you can use LDL-uptake potency of the cells, by using LDL-conjugated fluorescent dye.