Hello, to everyone.
I have really high values of log2FCs for some of my genes in treated triplicates vs ctrl triplicates (data from bulk RNAseq). I want to be sure that this is not an artificial output derived from rlog function in DESeq2.
For example, I have a gene that has a log2FC of 11.48. By checking the counts after DESeq2 analysis in the ctrl triplicates I have a mean counts = 0 and in the treatment triplicates I have a mean of 207,60. I guess it's normal such a high log2FC, isn't it!? If anyone could confirm this, it would be great!
Thank you a lot in advance!