I am really confused about this step, every single paper i read have a different methodology.
Now i have samples of immunized hemolymph and i want to extract peptides from these samples.
first i did partial purification by centrifuge at 4c removing pellet and have supernatant and kept it in -20 until use.
Now the next step is using FPLC or i should using sep pack c18 as a preparative step for more purified samples?
I gather that you are looking for antimicrobial peptides. The best general guide I can think of is: J Michaek Conlon "Purification of naturally occurring peptides by reversed-phase HPLC", Nature Protocols, Vol 2, No. 1, 2007, pp.191-197.
Please look at the following below links which may help you in your analysis:
https://www.itqb.unl.pt/labs/antibiotic-stress-and-virulence-of-enterococci/publications/artigo%20da%20cecropin.pdf
https://www.ijcmas.com/vol-4-4/Pavlina%20Dolashka,%20et%20al.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083081/pdf/nihms594012.pdf
Thanks
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