I am currently working on a plant and am interested in possibly extracting individual phytochemicals, to be tested for activity. From literatures, thw chromatographic techiniques do not seen to be able to achieve my aim. Beside the colunm chromatographic technique what other method can i use to get physical samples of my individual phytochemicals?
Supposing am interested in finding out the effect of plant extract on the metabolism of a toxic enzyme which contributes to virulence of an orgnism, how do i design me experiment.
Please find the details of extraction:
Abdel-Moemin AR. To investigate the antioxidant properties of dietary flavonoids in human metabolism [dissertation] Belfast: The Queen's University; 2004.
Thesis Chapter 2-TO INVESTIGATE THE ANTIOXIDANT PROPERTIES OF DIETA...
A different method to separate and collect the components is preparative TLC
As mentioned above in combination with the open column chromatography,TLC is very important to check for the similar compounds before the bio-assays techniques.
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