I am trying to extract gDNA from culturable endophytes isolated from Gum trees but have not been getting good result from the protocol attached. The endophytes are pigmented.
I used mycelia of endophytes cultured in broth. After centrifuge of precipitation step, the DNA pellete was contaminated with dark colour (dark pellete). So I did not proceed further.
I than repeat the extraction but this time I ground mycelia in liquid nitrogen and use the same extraction protocol. I obtained white precipitated DNA pellet unlike the previous one. Then I dissolved DNA pellete in milliQ water. The NanoDrop readings were well below A260/230 and DNA yield was very poor.
If anyone/experts of endophytes can help me with DNA extraction advice.
I am using fresh mycelia of endophytes cultured in both broth and on solid agar. Both still hasn't yield good result.
DNA extraction protocol attached.