Winnifred Duk

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Questions related from Winnifred Duk

Which DNA barcode is good for native plant identification?

Which DNA barcode is best for identifying native plants? I'm currently using ITS and trnL, but I'm not getting enough resolution at the species level. For one plant, DNA amplified with both ITS...

03 April 2025 1,523 4 View

How can I improve taxanomic resolution of Eucalyptus spp using trnL and ITS barcode?

I am trying to identify Eucalyptus sp using DNA barcoding. I am using chloroplast intron and plant ITS as DNA barcode. The amplicons are 500bps approx. When I perform BLASTn search on NCBI using...

02 July 2024 3,084 0 View

Extraction of gDNA from Endophytes from Medicinal Plant?

I am trying to extract gDNA from culturable endophytes isolated from Gum trees but have not been getting good result from the protocol attached. The endophytes are pigmented. I used mycelia of...

01 September 2023 2,349 1 View

I attempted 16S PCR on herbivore fecal DNA however, none of my PCR product amplified. I used 16S, ITS, UniPlant and plastids primers. Need help?

There is no amplification. I don't know what happened. If anyone can help me with some advise.

21 August 2023 1,049 12 View