Ahmad Mitoubsi

5 Questions 4 Answers 0 Followers

Questions related from Ahmad Mitoubsi

Extraction of Chromosomal DNA from Schizosaccharomyces pombe?

I have been using this protocol to extract dna from fission yeast though it is not working. I am getting two bands that are very small in size. Can someone troubleshoot with me...

08 August 2018 1,916 0 View

Ligation of three mixtures?

I have 3 inserts one is 464 bp, 1173 bp and 713 bp. I am trying to ligate and amplify them using pcr. I have done a overnight ligation and found very faint bands on my gel. I try to amplify though...

01 January 2018 690 0 View

Fusion PCR protocol?

I am trying to fuze 3 different inserts together. I am looking for a good fusion protocol.

12 December 2017 3,616 2 View

Overlap extension PCR?

I am looking for a protocol using 3 dna pcr.

12 December 2017 4,544 4 View

I am trying to fuse 3 dna together and have over lap?

I am wanting to fuse 3 dna together and insert them in a plasmid. Is fusion pcr?

12 December 2017 1,617 0 View