Dear Sruthi Chovva:

Allicin extraction is not an easy task due to its instability. Perhaps the easiest way to extract it is:

1. Using a mortar and pistle, crush cloves of garlic and transfer the contents (including any resulting garlic oil) into a beaker.

2. Soak the crushed garlic and garlic oil in 20 mL of ethanol (per clove).

3. Cover the beaker with a watch glass and allow it to stand for half an hour (room temperature or slightly below).

If you want pure allicin now you have to purify it faster than it can degrade into other chemical species. Though some have used paper chromatography using a 3:1 hexane:isopropanol mobile phase I'd try flash chromatography with a similar solvent system. Remember that allicin is produced by the release of the enzyme alliinase and that the enzyme comes in contact with the substrate (alliin) only after the garlic has been "damaged" so you'll have to really crush the cloves good if you want a high production of allicin.

Dear Sruthi Chovva:

Allicin extraction is not an easy task due to its instability. Perhaps the easiest way to extract it is:

1. Using a mortar and pistle, crush cloves of garlic and transfer the contents (including any resulting garlic oil) into a beaker.

2. Soak the crushed garlic and garlic oil in 20 mL of ethanol (per clove).

3. Cover the beaker with a watch glass and allow it to stand for half an hour (room temperature or slightly below).

If you want pure allicin now you have to purify it faster than it can degrade into other chemical species. Though some have used paper chromatography using a 3:1 hexane:isopropanol mobile phase I'd try flash chromatography with a similar solvent system. Remember that allicin is produced by the release of the enzyme alliinase and that the enzyme comes in contact with the substrate (alliin) only after the garlic has been "damaged" so you'll have to really crush the cloves good if you want a high production of allicin.

thank you sir

hope you can find here

http://article.sapub.org/10.5923.j.fph.20140406.03.html