Hello researchers
Please can you tell me how can we extract alkaloids from plants? Are the extraction of alkaloids same as polyphenols?
https://agritech.tnau.ac.in/horticulture/extraction_techniques%20_medicinal_plants.pdf
https://doi.org/10.3390/plants9020242
https://doi.org/10.1016/j.jfoodeng.2013.01.014
In general, extract into methanol and dry the extract. Take the extract and extract with dichloromethane and water containing 0.1 N HCl. Most alkaloids will extract into the water as their chloride salt. Next, take the aqueous layer and make it basic, around pH 9 or 10, with ammonia. Extract again with dichloromethane. Most alkaloids will be in the DCM layer as their free-base. I use HCl and Ammonia because NH4Cl is volatile and can be removed in the freeze-dryer if the alkaloid of interest remains in the aqueous layer.
Hi,
These papers used a validated method to extract alkaloids from plant, soil and water.
https://doi.org/10.1016/j.scitotenv.2020.142822
https://doi.org/10.1039/C9RA05301H You have an overview of the method recovery and matrix effect.
I hope this helps!
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts