Does anyone have any experience with successfully extracting RNA from tissue stored at -20?
My lab recently received tissue samples which were stored at -20. The tissues are not stabilized with any reagents, and are a few months old. The intended purpose of the extraction is to perform a RNAseq library preparation, so high quality RNA would be needed.
Looking forward to hearing if anyone has done this.
Thanks!
Hello
Regarding the high quality requirements of RNAseq library preparation, I would not do that. It would be a total waste of resources. For most RNAseq, a RIN value of above 8 in recommended, although you may try to do some qPCR on them. The RIN value requirements for qPCR is around 5.
Good luck
You can try a separate additional sample, store for a week, than test if quality of RNA is good. I have always used either LN2 or -70 freezer or preferably fresh tissue
What about main proceduration?
which include
The tissue homogening in liquid nitrogen, and total RNA was extracted with Trizol, and cleaned by using the RNeasy kit.
This proceduring for mycelium of fungi.
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