Anchored PCR (A-PCR) for genome walking?

i am trying to identifies unknown sequence in nitrate reductase gene from chlorella vulgaris using anchored pcr. first i extracted the genomic DNA and i did single primer extension using 0.5 ug of...

05 June 2018 8,739 11 View

DNA and RNA extraction from chlorella vulgaris?

hello i am trying to extract DNA and RNA from chlorella vulgaris. first i tried the CTAB methods but i couldn't get high yields and the quality of DNA or RNA. I used the TaKaRa MiniBEST Plant...

03 April 2018 6,663 10 View

Overlapping pcr of 4 fragments?

hello i am trying to overlap four fragment through use of overlapping pcr: the fragments are CMV promoter (595bp), Kanamycine resistance gene (795bp), IRES (206), and EGFP (720bp). i designed the...

01 February 2017 9,201 5 View

Live modified organism?

hello i was reading this paper http://www.cell.com/trends/biotechnology/abstract/S0167-7799(15)00088-8 and they used the cas9 protein and guide RNA without use of plasmid. is it possible to call...

10 November 2016 661 13 View

Protein Electrophoresis and Blotting for RNA page electrophoresis?

06 July 2016 1,983 3 View

What happen to microrna after inhibiting translation of mRNA?

hello everyone i am curious about the fate of microrna after inhibiting the translation of mRNA can someone provide me with information about it thanks in advance.

03 April 2016 471 0 View

Construction of artificial microrna vector to target a gene?

31 December 2015 2,661 4 View

MicroRNA blast against miRbase?

Hello everyone I did an experiment using microRNA NGS and because of lack of funds we did the alignment of microRNA against zebarfish microrna and I got some novel microRNA. Now I want to...

11 December 2015 9,701 6 View

Does anyone know about the inhibition of microRNA?

hello I wanna ask about inhibition of microRNA expression using vector expressing microRNA sponge. i was looking for some reference about this subject and couldn't find. can someone help me?...

10 November 2015 6,587 5 View

For MiRNA knockout, should I use a bulged microRNA sponge or a perfectly complementary one?

hello everyone i wanna knockout a specific microRNA and i would use microRNA sponge and through my readings i found some researchers used bulged sponge and others used perfect sponge complementary...

10 November 2015 5,602 0 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View