19 Questions 32 Answers 0 Followers
Questions related from Najib Abdellaoui
i am trying to identifies unknown sequence in nitrate reductase gene from chlorella vulgaris using anchored pcr. first i extracted the genomic DNA and i did single primer extension using 0.5 ug of...
06 June 2018 8,774 11 View
hello i am trying to extract DNA and RNA from chlorella vulgaris. first i tried the CTAB methods but i couldn't get high yields and the quality of DNA or RNA. I used the TaKaRa MiniBEST Plant...
04 April 2018 6,703 10 View
hello i am trying to overlap four fragment through use of overlapping pcr: the fragments are CMV promoter (595bp), Kanamycine resistance gene (795bp), IRES (206), and EGFP (720bp). i designed the...
02 February 2017 9,241 5 View
hello i was reading this paper http://www.cell.com/trends/biotechnology/abstract/S0167-7799(15)00088-8 and they used the cas9 protein and guide RNA without use of plasmid. is it possible to call...
11 November 2016 699 13 View
hello we have in our lab Protein Electrophoresis and Blotting apparatus from biorad and I want to use it for RNA page electrophoresis. is it possible to use it? thanks for your help.
07 July 2016 2,043 3 View
hello everyone i am curious about the fate of microrna after inhibiting the translation of mRNA can someone provide me with information about it thanks in advance.
04 April 2016 510 0 View
hello i am looking to extract the 3'utr sequences of fathead minnow fish using the annotation file and the genome. http://www.ncbi.nlm.nih.gov/genome/?term=fathead%20minnow can someone help me or...
03 March 2016 6,507 0 View
hello I am trying to target a gene using artificial microrna technology. I am planing to put the artificial microrna on the 3'utr of EGFP, the transcription will be done using cmv promoter and...
01 January 2016 2,701 4 View
Hello everyone I did an experiment using microRNA NGS and because of lack of funds we did the alignment of microRNA against zebarfish microrna and I got some novel microRNA. Now I want to...
12 December 2015 9,764 6 View
hello I wanna ask about inhibition of microRNA expression using vector expressing microRNA sponge. i was looking for some reference about this subject and couldn't find. can someone help me?...
11 November 2015 6,623 5 View
Hello everyone I am doing a research on olive flounder and I got a set of genes which are influenced by microrna. I want to do a gene ontology for this set of genes and I want to do also a pathway...
11 November 2015 7,935 5 View
hello everyone i wanna knockout a specific microRNA and i would use microRNA sponge and through my readings i found some researchers used bulged sponge and others used perfect sponge complementary...
11 November 2015 5,645 0 View
hello i have NGS data of microRNA at different time with 0 hour as control. the library construction and data analysis was done by a company. i want to know how to normalize the data?
08 August 2015 6,129 6 View
hello i am doing a research on microrna and i used the stem loop qRT-PCR for quantification of microrna. i designed the RT primer following the guidelines in chen et al paper. for the RT-PCR i...
05 May 2015 9,373 8 View
hello i am trying to do qPCR for a specific microrna from a virus and i want to confirm the mature microrna and also the pre-microrna. so i want to know how to designer primer to amplify the...
03 March 2015 9,051 2 View
I like to profile circulating microRNA from serum using NGS after exposition of animals to a toxic. For the extraction of microRNA from serum I am going to use QIAGEN mirneasy serum/plasma kit and...
02 February 2015 1,328 5 View
hello I just have the sequence of precursor-miRNA and the organism I am working on doesn't have its genome published. I looked for several days and couldn't find it. So my question is whether...
01 January 2015 5,933 10 View
Hello I am trying to clone the G gene from Viral hemorrhagic septicemia virus. For this purpose I cloned the G gene into pcDNA vector. After that I transfected it into EPC cell line and check the...
12 December 2014 10,007 4 View
hello i wanna know if it is possible to know the free energy for a given sequence (as example EGFP sequence) and if there is a software to calculate it.
12 December 2014 2,451 1 View
