What could be the reasons of protein not expressing in Bacillus Subtilis and Bt??
using vectors with autoinducibe promoters(Pylb and cyt1). I
have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
Libor Krásný
Just a couple of ideas:
1/ B. subtilis might have recombined your sequence. My experience is that it works much better when the construct is integrated into the genome [e. g. in the amyE locus] than when it is on a plasmid.
2/ B. subtilis has quite a number of proteases, using a strain with their deletions might help.
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