Is there a paper on the development of the SQFM(AA), the Shorter questionnaire for family members affected by addiction?

I can't find anything on the validation of this tool. It measures stress/strain/coping/support for family members of substance users (or those addicted to gambling).

23 January 2021 1,463 4 View

Is anybody familiar with technicalities of Google Scholar?

Many researchers have reported that their name is not findable (no hyperlink) via Google Scholar while their account is set on "public", the name does appear in the collaborator lists of...

10 September 2020 7,553 2 View

Can lead toxicity cause diabetes?

Can high exposure to lead oxidise pancreas beta cells, reducing insulin secretion? If so, what type of diabetes would this be?

30 June 2020 2,455 2 View

Sample size of horses for a pilot study testing reliability?

How do I calculate my minimum sample size (number of horses) for a study testing reliability of an outcomes measure for epaxial muscle activity?

04 March 2020 7,842 3 View

How to calculate SNR using BER?

Am working in Non-Orthogonal Multiple Access NOMA. I have doen the matlab code for power domain NOMA. I have found the BER too. Now i need to plot BER v/s SNR graph. Is there any equation...

27 September 2019 9,662 3 View

Why does improving interfacial recombination improves VOC in solar cells?

Many literature reports on the increase in VOC when interfacial recombination is being suppressed, with little change to JSC and FF. How can we explain this strong relation between VOC and...

27 April 2019 8,063 6 View

How can oxygen be incorporated into SnS using ALD?

I have successfully deposited SnS thin films using ALD by alternating doses of a tin (ii) metal precursor gas with hydrogen sulphide gas. For some oxygen to be incorporated into the SnS material,...

14 April 2018 5,595 5 View

Blurry PVDF membrane with no visual bands after imaging?

Hello everyone, I've been experiencing a problem with my western blots recently. For some reason my PVDF membrane is extremely blurry after imaging with barely of my bands showing up. I've been...

17 January 2018 2,307 3 View

Single/Double Exponential Decays in Time Resolved Photoluminescence

In the time-resolved photoluminesence measurements of semiconductor materials, what causes single exponential decays and what causes double exponential decays?

13 July 2017 5,480 4 View

Can anyone tell me why there rarely are any good antibodies for IHC receptors?

Hi, could anyone tell me why there aren't any good antibodies for receptors?

17 June 2016 3,674 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View