Dear Snehal D. Ganjave.Ph.D

i tried it some years ago, by shiftinig down the temperature at 32°C just after transfection (ad suggested for CHO) but it is not worked so well since differently to the CHO, which continue to grow even if slowly, at 32°C the metabolism of Expi293 seems to be more influenced by the temperature lowering and at the end i was able to recover very low amount of cells abd purified antibody. After some preliminary trials we decided to more on the ExpiCHO system which work very well at 32°C . Maybe You can try to do an itermediate approach as lowering down the temperature, not immediatelly after transfection but after 16-24h when you add the enhancers, but i never tried this way.

good luck

Manuele

Thanks Manuele Martinelli . Even I thought of lowering it to 32 deg after 24h of transfection.