Hi Estherina Trachtenberg

I briefly wanted to reach out concerning your inquiry. For a detailed flow cytometry protocol (with antibody (flourophore combinations and clones), cocktails, gating strategies and an analysis example please check:

Article Development of a High-Color Flow Cytometry Panel for Immunol...

And for a quick 'n dirty protocol for high-throughput analysis of a large populational screen in rats (with specific emphasis on T cells including their functional characterization), please check:

Article Self-reactive T cells induce and perpetuate chronic relapsin...

Article Natural Polymorphisms in Tap2 Influence Negative Selection a...

I hope that helps.

All the best & good luck for your experiment,

Michael

@Michael Förster

Thank you for your answer! Sorry for the late reply.

I'm familiar with the first article, but the two others are new to me

Thank you!