Has anyone used the product TaqMan ® Exogenous Internal Positive Control Reagents in multiplex real time pcr assay with good results?
We need to get the bacteria from the blood of patients with sepsis for diagnosis, is there any commercial device or prototype capable of efficiently purifying bacteria from blood sample (5-7ml)?
11 December 2016 8,633 4 View
Does anyone know a chromogenic carbapenemase substrate whose hydrolysis could be directly monitored in the visible wavelength range?
04 May 2013 1,548 1 View
Does anyone know any cell line containing integrated HPV16 genome?
11 December 2012 693 7 View
I'm interested in doing an Ultra deep sequencing experiment. Specifically I would like to sequence amplicons of 400 bp size with the 454 and I would like to know how many reads as a minimum people...
09 October 2012 4,594 2 View
FMT is showing promising results in terms of treatment of recurrent gut infections. Could be used this approach (microbiota transplantation) for the management of chronic respiratory diseases...
01 January 1970 1,632 11 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View