How to estimate charge transfer and polarization resistance in a battery?

Hi. I have performed many EIS experiments using a cylindrical battery at different temperatures and state of charge. Some results (near SOC=0) are as the attached figure. How can I find the...

08 July 2019 1,746 2 View

Are there different types of Dansyl Chloride?

I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I...

24 June 2019 1,484 3 View

Sputtering reactive can damage the magnetron?

I'm going to use oxygen as the reaction gas, but I'm worried that it will cause magnetron oxidation

08 January 2019 7,363 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

20 December 2018 3,718 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

11 December 2018 1,592 3 View

What is happening with my Cary Uv-Vis 100 spectroscopy equipment?

Good afternoon I have a problem with a Uv-Vis-100 cary spectroscopy equipment, In summary, today I wanted to measure and the signal appeared as the image that I attached, does anyone know what is...

14 October 2018 651 3 View

Loss of internal standard in a LC ESI MS/MS protocol for Gliclazide?

We are performing an LC ESI MS/MS protocol to quantify Gliclazide on plasma samples. We have noticed that the internal standard (Gliclazide D4) has a very high variation compared with gliclazide....

22 July 2018 8,936 4 View

What is half-life of mRNA of Tbet, AHR, and RORc?

01 February 2018 3,654 0 View

Do anyone Know if there is some procedure to perform Lumsdaine and Papel test for unit roots breaks in stata or gretl?

In RATS you perform LPUNIT procedure. I want to know if I can do in other statistical software

27 September 2017 4,208 2 View

How can I align images in a z-stack in image j in order to make reconstructios of biocytin labeled neurons?

I took pictures of some neurons I want to reconstruct using ImageJ; however, they are not in the same x and y plane. I can built a z-stack without problems, but of course, they do not appear...

20 June 2017 7,704 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View