How can I detect IL-1B by Western blot?

Hello Everyone, I know IL-1B is a soluble protein. So how to extract it from the supernatant? Please advice. Thanks!

05 January 2021 7,349 5 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Are there any EEG facilities for hire?

I need to run a quick EEG experiment. I don't want to spend time and money to set my own lab. I wonder if there exist EEG labs that can run an experiment for me for a fee?

10 September 2020 5,492 3 View

How do you report undetermined Ct values?

I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...

29 July 2020 8,198 3 View

Use of spherical silver?

Where is silver in spherical shape used other than for solar cell electrodes? Most of the silver in electronics seems to be flake. Is this statement accurate?

24 May 2020 7,604 3 View

Insect Population Counting and Studying Traps - recommend a good book or source for info on?

Hi, I'm looking for an entomologist that can recommend a good book on the trapping of insects for study. Thanks

30 January 2020 2,710 2 View

IHC: Monocyte vs Macrophage?

How would you distinguish peripheral monocytes from tissue macrophages with IHC? Specifically, I am interested in looking at rodent brain tissue. Any help is appreciated! Thanks, Dan

07 October 2019 2,683 3 View

Where can I find data on urban land cover in the UK from the 1960s?

I'm modelling insect populations over time (1968 - present) in the UK at over 500 sites. I have detected a decline in sites that are mainly suburban (based on 500m radii using the CEH Land Cover...

02 September 2019 535 3 View

Would (groundwater) dissolved noble gas concentration change after isolation from gas exchange with air?

Dear scientists of noble gas and hydrology, Would equilibrium and/or excess concentrations of noble gases change after isolation from gas exchange with atmosphere? (Non-atmospheric noble gas was...

20 February 2019 255 5 View

Is there a way to control for categorical groups to calculate partial correlations for various measurements?

I have a sample size of 30, representing three different groups (one intervention and two controls). I would like to see if there is an association between my dependent variable and 4 self-report...

18 February 2019 4,619 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View