This publication may steer you in right direction: Methods Mol Biol. 2010;618:359-69. doi: 10.1007/978-1-60761-594-1_22. Ex vivo skin infection model. Rubinchik E1, Pasetka C.

thank you

here is a model for host-bacteria interaction

 J Invest Dermatol. 2000 Jun;114(6):1120-5.

An experimental model of cutaneous infection induced by superantigen-producing Staphylococcus aureus.

Mölne L(1), Tarkowski A.

Author information:

(1)Departments of Dermatology and Rheumatology, Sahlgrenska University Hospital, Göteborg, Sweden. [email protected]

Skin infections caused by Staphylococcus aureus, such as erysipelas, are commonly occurring, painful, and costly for society. Despite the high prevalence of this condition, little is known about the host immune responsiveness and bacterial virulence factors during S. aureus dermatitis. We present here a mouse model of infectious dermatitis in which S. aureus is inoculated by an intracutaneous injection to the shaved back of NMRI mice. Visible skin inflammation, characterized by redness and swelling, was noted 48 h after inoculation of staphylococci in mice that received 2 x 108 colony-forming units of S. aureus. Microscopic evaluation revealed a dermal and subcutaneous infiltrate rich in macrophages and neutrophilic granulocytes already within 6 h after inoculation. A sparse influx of T lymphocytes was noted somewhat later. Bacterial cultures from skin revealed high numbers of staphylococci early after inoculation, with a successive decline during 2 wk follow-up. Total white blood cell count as well as the number of polymorphonuclear leukocytes peaked 2 d after bacterial inoculation. Also, serum interleukin-6 levels peaked within 2 d, with a 10-fold increase compared to non-infected control mice, indicating a systemic reaction to skin infection. The role of toxic shock syndrome toxin 1 in the pathogenesis of the dermatitis was assessed using isogenic S. aureus strains. Even though the gross inflammatory skin reaction was similar for mice infected with either of the strains, it was apparent that bacteria secreting toxic shock syndrome toxin 1 preferentially triggered influx of T lymphocytes to the skin. In addition, mice inoculated with staphylococci producing toxic shock syndrome toxin 1 showed a weight decrease during the experiment whereas mice inoculated with the isogenic strain showed a weight increase. This model of staphylococcal dermatitis will enable future in-depth studies regarding the host-bacterium relationship.

Here another one for infection drug testing

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 2005, p. 3435–3441 Vol. 49, No. 8

Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Establishment of a Superficial Skin Infection Model in Mice by Using Staphylococcus aureus and Streptococcus pyogenes

Elisabeth Kugelberg,

1,2† Tobias Norstro¨m,3† Thomas K. Petersen,4 Tore Duvold,4

Dan I. Andersson,

1,2 and Diarmaid Hughes3*

Department of Bacteriology, Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden

1; Microbiology and

Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden

2; Department of Cell and Molecular Biology,

Box 596, The Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden

3; and LEO Pharma,

DK-2750 Ballerup, Denmark

A new animal model for the purpose of studying superficial infections is presented. In this model an infection is established by disruption of the skin barrier by partial removal of the epidermal layer by tape stripping and subsequent application of the pathogens Staphylococcus aureus and Streptococcus pyogenes. The infection and the infection route are purely topical, in contrast to those used in previously described animal models in mice, such as the skin suture-wound model, where the infection is introduced into the deeper layers of the skin. Thus, the present model is considered more biologically relevant for the study of superficial skin infections in mice and humans. Established topical antibiotic treatments are shown to be effective. The procedures involved in the model are simple, a feature that increases throughput and reproducibility. This new model should be applicable to the evaluation of novel antimicrobial treatments of superficial infections caused by S. aureus and S. pyogenes

thank you

i need to make a superficial skin bacterial infection in mice or rat, have you any suggestion about how to inoculate the bacteria local in the skin? 

To Aml Imam Mekkawy :

Honestly added:

Please read at least the abstracts of the two papers as mentioned in Re[003] by Amar Safdar , e.g.,

i) '...inoculated by an intracutaneous injection to the shaved back of NMRI mice.' and - contrary to that 'infection by injection' - :

ii) '... In this model an infection is established by disruption of the skin barrier by partial removal of the epidermal layer by tape stripping and subsequent application of the pathogens Staphylococcus aureus and Streptococcus pyogenes. The infection and the infection route are purely topical, in contrast to those used in previously described animal models in mice, such as the skin suture-wound model, where the infection is introduced into the deeper layers of the skin. Thus, the present model is considered more biologically relevant for the study of superficial skin infections in mice and humans. ...'

I am suggesting one model in human for your consideration

Take patients with gingival enlargement

Infect the tissue of gingiva

Take out the gingival tissue by excisional gingivectomy

Examine histologically

Consider ethical issues locally