In particular if infrared spectroscopy is applied to organic or biologic materials, absorbance spectra are usually not processed by dispersion analysis or Kramers-Kronig analysis to obtain the true absorbance from the apparent/measured absorbance (i.e. -lg T or -lg (R/R0), where T is the transmittance, R the reflectance and R0 the reflectance without sample). If you apply infrared spectroscopy without further processing of the spectra by a dispersion or Kramers-Kronig analysis I would like to learn why. Is it because it is too time consuming or too complicated or because you think it is not necessary? Would you do it if it were automated and fast (say, less than a minute)? Please let me know your opinions.
Dear Krzysztof,
first of all, many thanks for your answer! Actually, I was talking also about measurements on thin films... a very recent example are transflection measurements of tissue on highly reflecting substrates which are interpreted without any processing, probably because the missing of too obvious interference fringes obscure what is going on.
We plan to create an algorithm which allows a fast one-click processing of transflection spectra, but I foresee that other thin film spectra as well as measurements of liquids in thin cuvettes would be treatable in virtually the same way.
Concerning your ATR measurements in the FUV and DUV region, I would assume that the oscillator strenght is usually much stronger compared to the infrared spectral range, very much the same way as for inorganic materials in the infrared, where processing is mandatory, otherwise the strong band shifts and band shape changes would render any interpretation meaningless. Therefore I can imagine that processing of the spectra is inevitable in this case.
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