We have two Native American undergraduates interested in chemical profiling of plant species with potential medicinal values. Can you share what methods you have been using for chemical profiling of plants? We would appreciate if you could recommend or share the relevant papers. Thank you in advance.
Data Phytochemical extraction and antimicrobial properties of dif...
Madhav Nepal ,
For chemical profiling of plant samples, various chromatography and spectroscopy techniques can be employed. As it involves the profiling of a plant, do not limit it to a single technique. Each techniques will yield a range of chromatograms with useful information to refer to. Some of the techniques that you can include are TLC, HPTLC, GC-MS, HPLC, LC-MS, 13C NMR. In addition, you can also include in the Chemometric analysis. It is an ideal tool to classify the spectroscopic data from whole plant extracts whereby it can be used to differentiate plants according to species, origin, processing treatment, age, and other quality parameters. These techniques have been widely applied to profile the chemical composition of a herbal medicine. Some of the references that you can refer to:
1) Article Chemical profiling and histochemical analysis of Bupleurum m...
2) Article Comprehensive chemical profiling of gramineous plant root ex...
3) Article Chemical profiling and chemical standardization of Vitex neg...
4) Article Chemical profiling of an antimigraine herbal preparation, ti...
Its long process where first of all if you know the plant and the species then you can have some idea that the plant belongs to some family have certain compounds in relation to the species. Then you need to screen the compound through different methods like TLC, HPLC , LCMS and NMR. The compounds which are known can be identified easily, but the compound may be new then, its a lengthy process. First you need to identify, purify, structurally elucidate it. Following references may help you ?
https://www.sciencedirect.com/science/article/abs/pii/S0031942211005644
https://link.springer.com/book/10.1007%2F978-3-540-74603-4
Regards
It has a great dependency on your constitutes of interest and analytical technologies are in high pace of advancement, of the highly capable ones: UHPLC, 2&3 NMR (C&H)as well as LC-MS systems to the best of my knowledge are the most frequently in use and reliable analytical systems. Additionally, comprehensive metabolic profiling projects are chiefly involve ecological and pathological studies with major concentration of plant defense systems.
For chemical profiling alone, none are better than chromatography-mass spectroscopy technique. It gives all known compounds in simple analysis provided there is a good library. GC-MS is good for volatile compounds, while LC-MS is suitable for non-volatile compounds. So if you run one sample with both GC-MS and LC-MS you will get a fairly comprehensive chemical profile.
- Badges
- Science topic
- Similar topics
- Papers