Which kind of HPLC column can be used for ethanol detection in LB medium after bacteria growth 24 hours?
You can use a reverse-phase HPLC column, after filtering-out any particulates from the bacteria, and broth medium, using a water:acetonitrile mobile phase, and UV detection at 210nm.
The most suitable HPLC column for detecting ethanol in Luria-Bertani (LB) medium after 24 hours of bacterial growth would be a reverse-phase C18 column. The HPLC method can reliably quantify the ethanol concentration in the LB medium after bacterial growth, despite the complex matrix composition and potential metabolic consumption of ethanol.
I agree: most of the separations I performed were on C18 reverse-phase columns. The ethanol peaks would be sharp and well-defined.
For detecting ethanol in LB medium after 24 hours of bacterial growth using HPLC, a reversed-phase C18 column with a suitable mobile phase (e.g., water and methanol/acetonitrile) is typically employed. Sample preparation involves removing bacteria and particulates, followed by dilution or extraction. UV-Vis detection is common. Optimization may be needed for best results.
Thank you very much. Dr Balamurugan Krishnamoorthy , Zoë Gaffen and Abdelhak Maghchiche .
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