A number of GnRH agonists will suppress estrogen secretion for weeks or months, depending on the type and route of administration. It will also suppress secretion of many other gonadal hormones. It would work much like ovariectomy. Drugs used to treat some forms of breast cancer, such as Tamoxifen, are strong estrogen antagonists and could be used as well. Tamoxifen binds to the estrogen receptors alpha and beta -- it is a partial agonist and partial antagonist for alpha and a strong antagonist for beta.

Three types of molecules used to either inhibit the synthesis of estrogen or inhibit the activity of estrogens. They are aromatase inhibitors (e.g. anastrazole) that inhibit the conversion of testosterone to estrogen, so inhibit the synthesis of estrogens. Another type of compounds are selective estrogen receptor modulators (e.g. tamoxifen) which competitively inhibit the binding of estrogen to estrogen receptor alpha and beta and the 3rd type of compounds are selective estrogen receptor down regulators (e.g. ICI182780/fulvestran), a pure ER antagonists that degrades both ERalpha and beta.  I guess, aromatase inhibitors are the best compounds to decrease the level of estrogen in the blood.

I agree completely with both of the earlier answers to this question. However, a point to consider is that both the use of aromatase inhibitors and anti-estrogens will lead to diminished estrogen feedback at the hypothalamic and pituitary level, leading increased levels of LH and FSH, ovarian overstimulation and increased levels of ovarian androgens.

My conclusion from responders' comments is that; first gonadectomy (ovariectomy and orchidectomy) and then regular administration of anti-estrogen and anti-testosterone to minimize the production of estrogens and testosterone in both sexes.

The reason for 'double' steroid hormone repression is also to rule out steroid hormone productions from adrenal glands (When we orchidectomized- castrated- male rats, we still observed reasonable amount of testosterone production (at least about four weeks after castration).

It is rather remarkable that you still find testosterone in the serum of 4 weeks castrated male rats. This contrasts with an earlier paper in which we described the absence of androgens in the serum of castrated rats and mice and the absence of androstenedione in the medium of incubated adrenal glands from these species (van Weerden, W.M. et al. Adrenal glands of mouse and rat do not synthesize androgens, Life Sciences 50 (1992) 857–861). This indicates that it will not be necessary to add anti-androgens to your treatment schedules. Your results might be explained on basis of cross-reactivity of other compounds in the assay you used to measure testosterone.

Dear Colleagues,

Thank you for your responses and comments on the anti-estrogen therapy.

The question is updated according to your comments. I will be glad, if you could comment it too.

Thank you again in advance...

Mehmet

Hello, Mehmet

Hope you doing I will suggest you to Use 4-Vinylcyclohexene diepoxide a industrial chemical which selectively destroys ovarian follicles reserve (primordial and primary) to mimic human menopause within 30 days of its administration. There are many publication available on it.

If you need any further help on it please do contact. Good luck...... 

If you wish to completely block estrogen activity, another approach is to infuse estrogen anti-serum to neutralize endogenous estrogen. Or you may be able to immunize the mice with an estrogen conjugated to a foreign protein. We have immunized pigs against androgens, GnRH and growth hormone.