Can i use DTT during a Zn affinity chromatography?

I need to purify His6 tag containing protein and im using a Histrap that is been charged with Zn2+. Can i add DTT to my lysis buffer or the column will not tolerate it when i insert the lysate to...

12 October 2020 5,910 4 View

How can I solve a checkpoint file problem in Gromacs 2018.4?

Hello everyone, I am running a simulation for 500 ns but after 200 ns I get the following error: The input requested 100000000 steps, however the checkpoint file has already reached step...

16 January 2020 454 7 View

How to model Constant-Current Constant-Voltage Charging of Lithium-Ion Battery in Comsol?

I am trying to implement a constant-current constant-voltage charging strategy using the COMSOL standard lithium-ion battery model that comes with the chemical engineering module. The idea is to...

02 December 2019 9,563 3 View

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts)?

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts). Degassing takes place with 5 cycles of 1 minute degassing in vacuum and 1 minute...

18 November 2019 5,423 2 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

25 July 2017 338 7 View

How to check the observability of a LPV system?

Consider the discrete system x' = A(t)x + B(t)u y = C(t)x' + D(t)u The matrices A, B, C, D contain time-varying parameters (calculated by a second model) which are given in the initial state, but...

19 May 2017 2,813 1 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

01 February 2017 7,322 4 View

How do I calculate sensitivity of android acccelerometers?

Hello, I would like to make a sensitivity experiment on android built-in accelerometers (tri-axonal) an make a statistical analysis (correlation etc) in Matlab. My questions are: 1..What would be...

08 November 2016 9,127 1 View

Any suggestions for efficient Raji cell transfection?

Hi everyone. Has anyone worked with Raji cell transfection? I recently used AMAXA technology to transfect Raji cells with a CRISPR construct that resulted in a very poor efficiency (~1%). When I...

17 May 2016 5,476 4 View

Could Raman spectroscopy reveal the presence of Pt and Ru nanoparticles dispersed on a carbon powder?

09 November 2015 8,352 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Why almost all medical imaging research use Deep Learning instead of Image Processing?

Currently, I'm researching to detect Covid-19 using chest X-ray images. When I looked at previous studies found out that almost all the research studies on medical imaging use Deep Learning...

21 February 2021 2,902 12 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View