Disc Diffusion and MIC data contradicting?

Hello, I am having contradicting data from antibacterial activity of plant extracts against selected bacteria. Under Disc Diffusion Method, at 400mg/ml concentration, there is presence of...

01 February 2018 2,246 6 View

Probe Sonicator & Lysis of Plant's cells?

Hello everyone, I am doing plant extraction of Curcurma caesia and Pandanus amaryllifolious by using cold maceration method to obtain both organic and aqueous extracts. I wanted to probe sonicate...

09 October 2017 5,921 0 View

Does drying organic extracts under the fume hood changes its nature?

Hello guys, I am extracting leaves and roots from 2 different plants by using maceration method (organic ext- Chloroform: Methanol). I did rotary evaporated my samples and they tend to have...

09 October 2017 5,904 4 View

Partial growth of B. subtilis?

Hello guys, I am currently doing antimicrobial work with plant extracts using disk diffusion test from Cucurma caesia and Pandanus amaryllifolious, both aqueous and organic extracts with different...

09 October 2017 3,235 4 View

Dissolving Plant Extract in 4% DMSO and Sonication?

Hello guys, Currently i am having problems of dissolving my solid organic extracts in 4% DMSO. The organic extracts were prepared and extracted using Choloform:Methanol (1:1) and treated under...

08 September 2017 9,536 3 View

Freeze Drying Vs Vacuum Filtration of Plant Extract

Hello guys, currently i am doing a project on antibacterial effects of Pandanus amaryfollious (leafy parts) and Cucurma caesia (Legume parts). I have both aqueous and organic extracts of the plant...

07 August 2017 2,395 3 View

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07 August 2017 6,081 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View