Hello everybody!
I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on:
Rabino, I., and Mancinelli, A. L. 1986. Light, temperature, and anthocyanin production. Plant Physiol. 81:922-924.
I want to calculate also Chl a, Chl b and Carotenoids concentrations from this acidic methanolic extraction. So far, I have only found 100% MeOH equations to calculate concentrations of these pigments, separately, but not a single reference to calculate them from a MeOH:HCl 1% (v/v) plant extract.
I have read that "A657 values provide an estimate of Chl content" (Photoregulation of Anthocyanin Synthesis' VIII. EFFECT OF LIGHT PRETREATMENTS, Mancinelli, 1983), but I would like to have a more precise value for Chl a, Chl b, and carotenoids also, if possible.
I have recorded measurements of wavelengths between 300-700 nm.
I would be grateful if anyone helps me with that.
Thank you all!
Hi, you can find more information in this chapter (Chlorophylls and Carotenoids: Measurement and Characterization by UV‐VIS Spectroscopy by Hartmut K. Lichtenthaler, Claus Buschmann). There are formulas for calculating the concentration of chlorophyll (a; b) and total carotenoids (x + c), depending on the solvent used in the extraction.
Thank you, Filimon V. Razvan for your response. However, this article does not have the equations for a MeOH:HCl solution, which is what I am looking for.
Best wishes!
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