11 Questions 21 Answers 0 Followers
Questions related from Carlos Augusto Manacorda
Hello! I am trying to find out a protocol or research paper about how to apply phosphorus to the leaves of Arabidopsis plants by means of foliar spray. I need references about KH2PO4...
02 May 2024 5,265 0 View
I need to know how to store Arabidopsis roots for further analysis in a way that hair roots are preserved in their integrity, without degrading or collapsing, suffering from dehydration, etc. I...
01 March 2024 8,144 6 View
Hi all! In the literature, one find reports indicating [P] either on a Fresh Weight (FW) basis (e.g.: A Central Regulatory System Largely Controls Transcriptional... or dividing by Dry Weight...
04 April 2023 2,390 2 View
Hello everybody! I need to use Mg(NO3)2 which, in principle, is hexahydrate (x 6 H2O), but the vial was not very well closed for a long time so water drops are now visible inside the vial. I...
15 February 2021 9,251 0 View
Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....
26 January 2021 3,996 2 View
Hello everyone! I have been wondering whether one can lower the number of technical replicates in qPCR experiments. Most of us use 96-well plates that are easily filled when the number of...
22 January 2020 6,930 3 View
When investigating past events and in the case of more than one ("competing") explanation being given to understand a historical event, is it valid to apply Occam´s Razor? In the sense, not of...
08 January 2019 6,298 6 View
I am trying to find out whether SHAM treatment should be done adding the hormone to the hydroponic medium or by the contrary, applying a spray to the rosettes.
18 August 2016 7,423 2 View
Hi all! I want to treat hydroponically grown Arabidopsis plants without spraying them so I am thinking of applying hormones directly in culture medium. In particular, hormones I am working with...
02 June 2016 1,180 3 View
I am trying to investigate the effect of phosphorus stress on Arabidopsis plants grown in hydroponic media and found that (Conn et al, 2013) stated the correct PO43- concentration is 0.6 mM. Does...
24 September 2014 9,855 4 View
In papers written by (Peng et al, 2007, 2008) and (Kant et al, 2011), they use soil-based system to grow Arabidopsis and found that 10 mM PO43- is sufficient to provide enough phosporus to avoid...
24 September 2014 3,968 4 View
