Carlos Augusto Manacorda

10 Questions 21 Answers 0 Followers

Questions related from Carlos Augusto Manacorda

How to store intact plant hair roots?

I need to know how to store Arabidopsis roots for further analysis in a way that hair roots are preserved in their integrity, without degrading or collapsing, suffering from dehydration, etc. I...

01 March 2024 8,134 6 View

Plant Phosphorus concentration: relative to Fresh Weight (FW) or Dry Weight (DW)?

Hi all! In the literature, one find reports indicating [P] either on a Fresh Weight (FW) basis (e.g.: A Central Regulatory System Largely Controls Transcriptional... or dividing by Dry Weight...

04 April 2023 2,387 2 View

How to dry up Mg(NO3)2?

Hello everybody! I need to use Mg(NO3)2 which, in principle, is hexahydrate (x 6 H2O), but the vial was not very well closed for a long time so water drops are now visible inside the vial. I...

15 February 2021 9,248 0 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

26 January 2021 3,993 2 View

Prioritizing biological or technical replicates in qPCR?

Hello everyone! I have been wondering whether one can lower the number of technical replicates in qPCR experiments. Most of us use 96-well plates that are easily filled when the number of...

22 January 2020 6,922 3 View

Is the use of the Occam´s Razor justified in Historical Research?

When investigating past events and in the case of more than one ("competing") explanation being given to understand a historical event, is it valid to apply Occam´s Razor? In the sense, not of...

08 January 2019 6,296 6 View

Could Arabidopsis transport SHAM (salicylhydroxammic acid) from a hydroponic medium by using its roots?

I am trying to find out whether SHAM treatment should be done adding the hormone to the hydroponic medium or by the contrary, applying a spray to the rosettes.

18 August 2016 7,413 2 View

Is it possible to perform hormones treatments in hydroponic medium?

Hi all! I want to treat hydroponically grown Arabidopsis plants without spraying them so I am thinking of applying hormones directly in culture medium. In particular, hormones I am working with...

02 June 2016 1,177 3 View

What Pi concentration should be used for making a Pi-deficient media for hydroponics-grown Arabidopsis?

I am trying to investigate the effect of phosphorus stress on Arabidopsis plants grown in hydroponic media and found that (Conn et al, 2013) stated the correct PO43- concentration is 0.6 mM. Does...

24 September 2014 9,852 4 View

What is the difference between Pi concentration in hydroponics and soil?

In papers written by (Peng et al, 2007, 2008) and (Kant et al, 2011), they use soil-based system to grow Arabidopsis and found that 10 mM PO43- is sufficient to provide enough phosporus to avoid...

24 September 2014 3,964 4 View