Eosinophil gating by flow cytometry for Rhesus monkey blood ?

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George Robin Barclay

High side scatter helps - and eos are way out beyond anything else.

Fanny Legrand

Hey Rustom! Thanks for your response ;)

Yes, I read this paper, I ended up to try different antibodies, and we got very lucky!

because the siglec-8 (BioLegend) worked fine! but not the RD clone.

I haven't tried the VLA4, good to know.

I will update soon hopefully the list of clones I tried on the website:

http://www.nhpreagents.org/NHP/default.aspx

We did other stainings that needs to be confirmed, cell sorting coming soon.

We are also dealing with a weird eosinophilic rhesus monkey apparently..;)

Best,

Fanny

Please come and look to my poster on rhesus monkey I present next week to the AAI meeting in Austin!! We can isolate eosinophils from monkey blood!