Hi.
I have attempted to internalizing 2 um microparticles inside some suspension cells but find this task quite challenging.
I am using an angiogenesis chamber that can hold up to 60 ul, in which I put about 50K microdisks and 50k Jurkat cells.
It appears that Jurkat cells are capable of internalizing these microparticles without any problem upon they come into contact with the particles, but since they are not so much mobile, the uptake efficinecy is much lower than the efficiency I observed in adherent cells.
I tried to make cells more mobile and remove clusters by pipetting the cells up and down vigorously every 3 hour, but it does not improve much.
Do you have any advice or any paper that I can refer to?
One option would be to leave the tubes on a roller the whole time. This way, the cells and the micro particles are mixed continuously and will come in contact more often. If it does not interfere with your experimental objective, you could also try making the Jurkat cells more adherent by adding them to poly-lysine coated plates. The cells should stick to the plate better. Add the micro particles and spin the plate down (1500 RPM, 5 mins) to make sure the particles are settling on the cells. This should improve particle uptake. You could remove free particles later by washing the cells using PBS.
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