7 Questions 3 Answers 0 Followers
Questions related from Seung Hyung Lee
Hi, I've been using cryopreserved PBMCs from ATCC and iQ Bioscience and found it weird that in their manual, they have direction for thawing the cells but do not have re-freezing the cells. My...
26 November 2019 9,102 3 View
Hi, I was working on running flow cytometry using Jurkat cells that have uptaken 2 um silica beads. In order to facilitate delivery of silica beads into Jurkat cells, I coated the silica beads...
23 October 2019 8,252 0 View
Hi, I am curious on HEPES Buffer's effect on cells. Per protocol, I am supposed to use 25mM HEPES buffer, but what would happen if I use 1M HEPES instead? Will it affect cell viability? Thanks!
18 October 2019 1,324 2 View
10 October 2019 4,564 2 View
Hello! I have been working with Jurkat T Cells for flow cytometry. I noticed that although Jurkat cells are T cells, their positive signals for CD3 antibodies were not so high. I've used 4-5 CD3...
10 October 2019 7,331 7 View
Hello, I am currently using a very old flow cytometer and having an issue with its software. The software plots the data in a very set way, but I would like to make some modifications. Is there...
09 September 2019 1,738 1 View
Hi. I have attempted to internalizing 2 um microparticles inside some suspension cells but find this task quite challenging. I am using an angiogenesis chamber that can hold up to 60 ul, in which...
08 August 2019 4,885 1 View
