EMT by TGFb and SB?

24 January 2024 0 9K Report

Hi everyone, I am treating A549 cells with TGFb and SB431542 to follow the EMT by checking EpCAM and CD90. However, I am not sure the duration in which the cells should be treated in either one of the drugs.

How long should I keep the drug in medium if the treatment exceeds 5-6 days and do I need to replenish the drug in between?

Many thanks

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