I have a question too. I am trying to do electroporation of stimulated Primary CD4 T cells with GFP and another plasmid. If anyone has done Neon electroporation of primary cd4 T cells, what program (voltage, width, pulses) worked best for you?
plant design simulation.
18 March 2024 7,411 1 View
Specify absorber conditions and specifications and if possible flow rates of both the feed gas and solvent.
18 March 2024 9,500 0 View
For my research work, I want to find the Biomechanical hand force calculation for pulling and pushing of hand lever; kindly suggest similar research articles.
16 January 2024 1,527 2 View
I'm currently facing an issue in my ARDL estimation, the model has decided to use 0 lags for one of the variables, but it is not showing the short-run coefficient. Instead this is shown: Variable...
01 January 2024 3,248 3 View
A description of the readiness for the acceptance of PHV vaccine would be necessary in this world of preventive medicine.
08 November 2023 4,545 4 View
I'm learning Fibonacci Sequence in my University and I want to know how is it used for modern day technologies and businesses.
14 August 2023 2,130 3 View
I'm a freshmen and learning Information Technology on Philippines. I've heard that most developers uses Python. But others use JavaScript. I don't know where to start but they are reliable and...
02 August 2023 4,546 7 View
I am conducting qualitative research on teachers' perceptions about the impact of covid-19 pandemic on their performance. How do draft the survey questionnaires to collect the data. I need help...
23 April 2023 6,249 2 View
I am doing qualitative studies; can anyone help me with how to draft a questionnaire to collect information about the impact of covid-19 on teachers' performance.
23 April 2023 6,238 3 View
I want to know how you write a research proposal and particularly it’s introduction because it seems I am not allowed to write an entire introduction with only my ideas.
20 March 2023 2,602 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View