Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

How is deep learning applied in analysis of open-ended survey questions?

Hello, Can someone give me an idea on how deep learning applied in the analysis of open-ended survey questions? My intuition is that applying deep learning models for analyzing the vast...

07 December 2020 4,479 3 View

Doing a PhD research in Natural Language Processing from a statistics department...?

Hello, My situation is a little strange because I am a Statistics PhD student but I ended up doing research in Natural Language Processing (NLP)/ Deep Learning due to my supervisor's...

26 November 2020 7,293 3 View

Does good inferential comprehension first require solid literal comprehension?

Hello, This can be a silly question to ask, but my main field is not in reading comprehension. While I try my best to phrase my question, it can sound a bit awkward. Thank you for your...

11 November 2020 811 3 View

How are the 3 levels of reading comprehension reflected in grade-level English Language Arts curriculum?

Hello, How are the 3 levels of reading comprehension (literal, inferential, and critical) reflected in grade-level English Language Arts (ELA) curriculum? In particular, normally, is literal...

01 November 2020 1,240 4 View

Is the level of cognitive flexibility positively correlated to the level of reading comprehension?

Hello, Is the level of cognitive flexibility positively correlated to the level of reading comprehension (greater cognitive flexibility brings higher level of reading comprehension)? If there is a...

01 November 2020 8,254 5 View

What could be the problem in these pcr results?

All the pictures I have uploaded are gel image of genotyping. What I don't understand is, most of the time, I get very very faint second band, and I assumed it's probably a problem with primer...

28 October 2020 9,410 9 View

Is the Syntax-first model in sentence processing widely accepted, or is it facing opponents?

Hello, Is the syntax-first model of sentence processing widely accepted, or is it facing more opponents as time goes by? I know that a study in frontal-temporal network of human brain has...

23 October 2020 1,084 9 View

Is there a reading comprehension theory that suggests simple reading questions are best solved via simple text analysis, and vice versa?

My main field is not Linguistics/reading comprehension so what I am asking might sound a bit silly or not make a lot of sense. My question could certainly be better-phrased. Is there a theory in...

23 October 2020 8,310 2 View

PCR recipe with 2 reverse primers?

Should my forward primer volume in the mastermix should be same with other two reverse primers? or do I need to double the volume of forward primer so that the sum of volume of reverse primer 1...

12 October 2020 9,464 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View