Hi all!

I am currently trying to barcode hepatitis B virus DNA via PCR, but due to the complex nature of HBV DNA, my PCR results have been unsuccessful.

Through discussions with my advisor, we have came to the conclusion that the RNA primer attach onto the HBV plus strand DNA prevented effective repair with DNA polymerase and thus prevented barcoding with PCR.

So, I would like to ask for an effective method capable of completely remove RNA from a DNA-RNA hybrid.

RNase H1 and RNase H2 were considered but they will leave a single RNA nucleotide remained attach to the DNA, so we are still looking for a better alternative.

Many thanks!

P/S: A detailed figure of HBV DNA in Figure 1(a)

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