Dear community !
I am extracting sulfated polysaccharide from various algae and I would like to add an alkali treatment to some my extraction protocols (notably to remove certain impurities).
I have found that strong alkali treatment could cause damages to polysaccharides, which range of concentration of NaOH will start be significant ? (I know it will depend of the polysaccharide properties, but I would like to have an idea of which range it does for sure, and which range in does not to stay safe). Is 0.1M good ? 1M ? Other parameter such as time of contact temperature also play a role here. Would 1h at 1M at RT be enough to induce a lot of damage ?
Finally, some of the polysaccharides I am extracting are sulfated, would alkali treatment affects the sulfate group on the polysaccharide ? I can not find proper literature on the topic.
Thanks for your help,
Arthur
Dear Arthur Nils Robin
Yes, Alkali treatment should affect the sulphate linkage and even affect the
glycosidic linkage. One of my results from study also prove that I could not present data here(Unpublished work). I found decrease in sulphate content at 1 M acid and alkali even at 25 degree and exposure time 18 hours. Better compare the sulphate content after & before treatment with alkali.
Good luck
.
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