I tried to culture primary hASCs in alpha-MEM culture medium but I made a mistake to add more than normal amount of FBS; 30% instead of 20%. Do you think this is a critical matter? it affects cell growth or survival?
Airflow Turbulence Air Dynamics Signal effect frequency response
02 June 2024 2,986 1 View
except BSA and in printed screen electrodes
11 May 2024 1,449 2 View
The World Bank database has a bug! The share of value added of each economic sector to GDP (%) must be equal to the value added divided by GDP in a certain year and country. However, they are not...
10 May 2024 609 1 View
The data derived from the World Bank shows that the summation of the value added of different economic sectors (agriculture, industry, manufacturing, and services) is not equal to GDP! Why? A...
26 April 2024 9,544 3 View
Hi everyone, I established a murine spheroid for glioblastoma, and I want to fix and stain them to conduct immunofluorescences. I have tried several times, but the size of my spheroids are small,...
07 February 2024 793 2 View
As we know, in partial differential equations, we deal with pure mathematics or computational methods, so in what aspects, machine learning can help us to solve partial differential...
15 January 2024 1,951 2 View
TPD/TPR
04 January 2024 4,711 3 View
Hi everyone I imported some pictures in Matlab. Then, I made them binary. It is a matrix consisted of 0 and 1 . I want to convert the isosurf (volume) achieved from Matlab into STL. Can you help...
04 January 2024 3,554 2 View
Hi everyone Mycoplasma detection has been a major challenge for me lately. I do cell culture and use qPCR to detect mycoplasma. In addition to controls(+,-) and NTC, I use 3 different strains of...
03 December 2023 3,145 5 View
Heat or cold denaturation, which one is more irreversible? Why? Do you have a reference on this?
29 November 2023 7,319 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View