Dear all,
I am doing zetasize analysis of biogenically synthesized nanomaterials at 1mg/ml (standard concentration for DLS as mentioned in NCL guidelines). I was getting a fluctuating result every time even if keeping optimum tempearture, sonication time. Why is it so?
Secondly, when I increased the concentration to 3mg/mL, I got even better results with lesser particle size and less PDI upto 0.2. What may be the reason or cause. Kindly advise.