Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

20 January 2021 8,221 2 View

Does R always evaluate all parts of a complex logical expression?

I am curious about some details of work of logical operators in R. For example, let we have following expression: A == a & B == b & C == c & D == d &.... and so on. If A == a gives FALSE, will...

19 December 2020 7,296 7 View

How can I perform pairwise protein alignments using BLAST, but without search?

I have a set of about 1000 homologous protein sequences with high diversity across various genera and families. What I need is to perform BLAST alignment of each possible sequence pair and obtain...

25 October 2020 1,199 5 View

How to move chromatograms in bioedit to make them visible?

Hello everyone, I've started doing molecular biology, rather recently. So, I've spent some time to obtain my first sequences at ABI platform and all of it were perfectly readable wheteher seq....

06 November 2017 8,312 6 View

What combination of chemotherapeutic agents for trimodal therapy of bladder cancer (MIBC) are more effectives? Is resonable use MVAC?

24 February 2017 1,652 1 View

How can I deal with the bleaching effect in FTIR spectroscopy?

19 September 2014 1,377 17 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

Is there any special rule to find out possible room-temperature stable silicates' chemical composition, if not crystal structure itself?

Suppose one needs to find out room-temperature stable silicates chemical composition of a particular cation, suppose magnesium. Is there any rule to estimate which stoichiometric values of metal...

13 January 2021 7,385 4 View

How will AlphaFold change the Protein Structure Prediction problem based researches in the future?

11 January 2021 9,600 4 View

What is New Next Generation Attenuation Relationships (NNGAR) and how are they different from other attenuation relationships?

Dear researchers The provisions of ASCE 7-10 states that New Next Generation Attenuation Relationships (NNGAR) has been used in the process of Probabilistic Seismic Hazard Analysis (PSHA) to...

15 November 2020 1,132 4 View

Selection of Steel Frame Section in SAP2000?

Hello, I have a technical question about the selection of steel frame sections in the designing of steel frame structure i.e W16x48 etc. The common practice I have seen is the trial and error...

11 November 2020 5,762 4 View

Can anyone please tell how to define link for Tuned mass Dampers using Sap2000/Etabs?

Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...

11 November 2020 8,309 11 View

How can I generated Simulated/Predicted Effects from an MRQAP model?

I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...

05 November 2020 1,215 2 View

Sir can you send me your research paper " Earthquake risk management assessment and evaluation for turkey" in short time?

05 November 2020 10,019 3 View

List of peptide structure prediction services?

Hi all, I am interested in peptide structure prediction services, both secondary structure and tertiary structure. Protein prediction services that can predict structures of short peptides are...

30 October 2020 4,314 4 View