EcoNI ligation Using Quick Ligation Kit (NEB) ?

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Aamal Ghazi Mahdi Al-Saadi

Hello Hao,

I used to use the Reaction Conditions of

1X CutSmart Buffer and Incubate at 37°C for about 30 minutes.

Good luck

Michael J. Benedik

I presume you realize that because of the variation in EcoN1 sites, not all fragments will ligate to each other.

Benjamin THOHA Thomas

Yes but all fragment will not ligate to each other because of the differences in the EcoN1 sites