Kambiz Gilany

14 Questions 17 Answers 0 Followers

Questions related from Kambiz Gilany

Have anybody a list of secondary amino acids in order to see antioxidant capacity?

Hey everybody. I have done amino acids analysis by LCMSMS. I am looking for a list of secondary amino acids in order to see antioxidant capacity in these molecules. anyone have a such list???? TNX

13 June 2023 5,881 3 View

Can Raman spectra nbe used as quantification in metabolic fingerprinting?

Dear all, I am using metabolic fingerprinting for my clinical studies. I am in doubt that I can use the Raman spectra for quantification? Can I use it semi-quantify? Can you provide me with...

12 April 2022 7,782 2 View

How to calculate amino acids concentrations when I am using isotope labeled compounds as internal standards in LCMSMS?

Dear All. I have separated 38 amino acids on HILIC column couple to QQQ followed by using 10 isotope-labeled amino acids as internal standard. However, I am not sure which formula to use to...

29 December 2021 2,574 4 View

ECL versus CLIA?

Hi everybody. I am interested to know what is different between: electrochemiluminescence (ECL) method and chemiluminescent immunoassay (CLIA) method ? TNX... Kambbiz

17 December 2018 6,591 3 View

How to constructe metabolite pathway in seminal plasma?

Hej Everybody. I have catalogue metabolites identified from human seminal plasma. I want to construct metabolite pathway. I have used KEGG. However, most of the metabolite was not in the KEGG. Is...

03 February 2017 6,343 5 View

MALDI-TOF/TOF-MS: t2d converter

Hi fellows. I am looking for a t2d converter where I can send the data to MASCot or other software? Can you help me out. I have fund 1 but it can not be...

25 June 2016 7,181 3 View

Plasma proteome vs seminal plasma proteome. How can I do it in a smart way?

Hi Everybody. I have collected more than 2000 proteins from human seminal plasma. I want to compare it to the human plasma proteome. Is there any smart software out there which can help me...

13 June 2016 3,752 2 View

How many 2DE gels run for clinical proteomics trial?

Hej Everybody. I am looking for to get answer how many 2DE gels I have to run for a pilot study in clinical proteomics? Should I pooled the sample and run three 2DE-gels or just run 3 good...

14 May 2016 3,377 4 View

Analytical versus Preparative 2DE gels? What is differences?

Hi everybody. I have been study 2DE-MS long time. It has been send to me for MALDI-MS analysis. They are big gels. I have ran a few big 2-DE gel a long time ago. Now, I have the 2DE instrument. I...

11 May 2016 7,161 3 View

What can I do when the watergate of NMR does not Work?

Hi Everybody. I have problem with watergate of NMR for metabolomics study of Saliva. It does not work. I was looking for suggestion how to solve this problem! I read a place which was suggested to...

03 May 2016 7,740 2 View

Is someone familiar with metabolite identification?

I need help for metabolites identification from a ion trap GCMS. Can anybody help me out? I have done untarget metabolic profiling. The result look very good. Now I just need to identify the...

17 September 2014 6,486 7 View

2DE trouble shooting?

Hi everybody. In attachment you will find my latest running of a human plasma 2DE. I have run MARS14 before and the collected proteome profile is shown. Can any body suggested how I can improve...

01 January 1970 8,694 8 View

To pool or not to pool the huam proteome sample?

Hi everybody. I am a little bit confuse regarding to pool the sample or not to pool the sample regarding the human proteome sample I have. What should I do for 2DE analysis? Should I pool e.g. 6...

01 January 1970 797 3 View

What is origin of metabolite/metabolites?

Hi Everybody... I have a question in mind what is origin of metabolite/metabolites? Including primary or secondary metabolite? We know the information goes from DNA->mRNA-> protein? TNX

01 January 1970 6,589 2 View