I can't answer that but here are some

new Ebola diagnostic tools:

http://www.medgadget.com/2016/04/cellphone-sized-device-pcr-testing-ebola.html

http://www.medgadget.com/2015/10/microoptofluidic-chip-detects-ebola-faster-easier-than-pcr.html

http://phys.org/news/2015-09-chip-based-technology-enables-reliable-ebola.html

http://phys.org/news/2015-09-portable-rapid-dna-ebola-pathogens.html

http://phys.org/news/2015-08-paper-based-quickly-ebola-remote-areas.html

http://www.medgadget.com/2015/02/10-minute-test-diagnoses-ebola-dengue-yellow-fever.html

https://idw-online.de/de/news619685

http://spectrum.ieee.org/tech-talk/at-work/test-and-measurement/printed-dots-detect-ebola-and-more-without-a-lab

http://www.nature.com/articles/srep14494

http://jvi.asm.org/content/78/8/4330

Regards,

Joachim

Hi Joachim and thanks a bunch:)

Best,

Joel

Prof Kou Hayakawa given detailed information on EVD,diagnosis.

Virus isolation by cell culture,detecting the viral DNA by PCR,detecting proteins by ELISA are the methods.In 2015,WH0 approved rapid antigen test,gives results in 15 minutes.Reliable test to confirm 92% with affected and rule out 85% not affected..You may download my paper on EVD,Doi::10.9790/0853-150405142148.Rgds..Murtaza

Hi and thank you Kou and Murtaza for your answers/info:)

Best,

Joel

Welcome

Murtaza

 To Murtaza and Kou,

what would be the count in serum during an Ebola diagnosis when symptoms do not appear and this count is too low to be detected?

Best,

Joel

Hi Kou and thank you for the information.  For just Ebola virus what level of serum protein or nucleic acid from Ebola can not be detected?  Meaning just before symptoms appear in the patient anything lower than x ug/mg can not be detected, what would be x?

Joel

 Hello Kau,

thank you for this valuable info,  (I am wokring on diagnosing Ebola in vivo via theoretical Molecular Dynamic Simulation via Nanoreceptors/sensors, hence my questions.)

In your first email you mentioned "PDMD method" what does this stand for?

Best,

Joel

Hello Kau,

in your first email you mentioned " at 3.0 ug/mg of serum protein in the healthy 33 y male, who is a attending physician of this gait disorder patient."

Please tell me for the above, when do symptoms of Ebola usually appear ie. how many ug/mg?  (I understand this may vary per patient so what would be this variance?)

Best,

Joel

Hello, reference towards your first response 'Therefore, Malaria protozoa of Plasmodium falciparum seems to be the origin of ebolavirus infection. "

Ide imagine that you are inferring that ebola co-infected with malaria via unsanitary areas and a lowered immune system correct?  If not explain?

Thanks:)

Joel

Kau,

I am assuming the vector for the co-infection of both Ebola and Malaria was via a monkey.

Joel

Thanks Kou:)

Best,

Joel

Hello and thank you a bunch for this valuable information Kou, duly noted:)

Best,

Joel

Thank you Kou for this information:)

Best,

Joel

Hi Kou and thank you for this new information:)

Best,

Joel

Hi and thank you Kou for your information:)

Best,

Joel

HI and thank you Kou for this information!:)

Best,

Joel

Hi and thank you Kou for this information.:)

Best,

Joel

Thank you Kou.

Best,

Joel

Thank you Kou.

Best,

Joe

Thank you Kou.

Best,

Joel

Hello Kou very interesting comments, thank you:)

Joel

Hello Kou,

I will be beginning my Doctoral Dissertation in January 2017 covering the detection of Ebola virus in vivo via a nanorobotic vehicle diagnosing Ebola while in the bloodstream and capillaries of infected organ systems.  My plan of design is to detect Ebola antigens, viruses and its RNA while they are are at an extremely low abundance.  Which of these is the most prevalent in vivo and where?

Thanks:)

Joel

Excellent

Dear Joel

Please check the following publication: Rapid Diagnosis of Ebola Hemorrhagic Fever by Reverse Transcription-PCR in an Outbreak Setting and Assessment of Patient Viral Load as a Predictor of Outcome. J Virol. 2004 Apr; 78(8): 4330–4341.

Hoping to be helpful

Marius

HI and thanks a bunch:)

J

Hello Kou,

are you familiar with this answer....what is the best treatment for EBola virus victims?

My suggestion would be to test patients spleens for EBola before they become symptomatic and if positive inject their spleens. livers and venous systems with substantial amounts of convalescent antibodies for Ebola.  Would you agree?

Joel

Hello Kou,

please tell me, although each patient is different, once a patient shows symptoms of having EBola how many days do they usually live from day 1 of symptomology?

hello Kou and thank you for your reply,

from my research the Ebola will firstly infect the organs ie. spleen, liver, lymph and subsequent to damaging these organs it will move to the immune/venous system to damage blood vessels via an immune system damage conduit.  Accordingly, my best therapeutic would be to diagnosis the spleen and or liver to everyone in an outbreak area before symptoms appear via biopsy of the spleen and or liver.  If someone is positive then sleep, IV fluids, and convalescent antibody treatment before symptoms appear since the virus count in the blood will be expected to be quite high shortly after the pre-symptomatic stage.  

The Ebola blood test is only specific and sensitive once the count is high after patient symptoms first appear, unfortunately due to the rapid progression of the Ebola, treatment post symptoms will probably experience a much higher mortality rate than pre-symptom treatment.  Would you agree?

Joel

and my point was not about prevention it was about Ebola survival, ie. once a patient has Ebola their is a high percentage for the patient's mortality.  Accordingly my belief is it is best to manage the patients survival via the earliest diagnosis possible.  

Current diagnostics techniques for Ebola are not only not 100% sensitive and specific but can only detect Ebola after Primary symptoms occur, many time this is too late for the patient's survival due to the rapid progression of Ebola.  My treatment decision is a bombardment of convalescent antibodies BEFORE symptoms appear to try and arrest the Ebola once it enters the blood stream.

Would you agree?

Hi and thank you for your prompt insight,

however in this Ebola case I think that convalescent antibodies is the only choice due to Ebola's extreme virulence and high mortality rate.

Joel

Hello Kou,

when a patient is first infected with Ebola my understanding is that there is not really a primary viremia and for the first week Ebola just sits in the organs and or white blood cells nesting.  Subsequent to this ~4-7 day stage ebola sheds into the circulatory system causing a full blown viremia that grows in time proportional to it mortality rate.  This is when there are symptoms and antibodies, antigens, and virus to detect for diagnosis.  

I also understand that there is a superficial primary viremia that occurs before and during ebola's nesting stage where there are 1-3 Ebola epitopes/antigens and human antibodies for this in vivo but very very diluted and therefore difficult to ascertain via current ex-vivo diagnostic techniques.  

1.)  What are these epitopes and antibodies during the primary viremia?

2.)  How dilute are they in vivo, meaning what is there (and any Ebola particle) count during this primary stage?

3.)  What is the minimum count for these to be detected via best current diagnostic techniques?

Thanks:)

hello and Happy New Year to you too and sure no problem:)

Hi and thank you Kou for the update:)

Joel