does anyone know if the inactivation of E. coli-GFP at 65 ° for 30 min, maintains its fluorescence and how effective is it to kill the bacteria? and if they have other methods to kill them and preserve the fluorescence?
What I want to do is put the bacteria in a phagocytosis assay and I prefer them death to avoid contamination in the incubator
Michael J. Benedik
most strains of E. coli will die at lower temperatures, try 30 min at 52C and you can do a quick viability count afterwards. My lab strains are always dead under those conditions. There are also many chemical means of killing the bacteria such as azide, although you would need to remove before using in your assay.
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