Hi everyone,
With the weird social isolation issues we are all facing i am hoping to find a few people to start some discussions.
If we can't attend conferences, then why not try some other means of trading some knowledge to help get our experiments moving again when we are back in the lab (if you find yourself in isolation that is).
I have a project at the moment looking at epithelial mesenchymal transition in CF airways. I am a little bit stuck at the moment after developing a lineage tracing vector, which possibly stems from not enough fore thought regarding E and N-Cadherin gene regulation.
If anyone has any experience with EMT, E or N-Cadherin gene regulation, or lineage tracing vectors/methods, it would be great to chat to you.
Happy to discuss via email and or zoom
Kind regards,
Nathan
Thanks for the share Suhad. I am really looking for more detailed understanding of E-cad and N-cad gene regulation. I have created E-cad and N-cad KO cell lines in hope that it would shift the cells towards the opposite side of the spectrum. What i am not sure about though, and what i suspect is not occurring, is that if i then add a gene (using a plasmid) with a promoter for the knocked out gene, will i ensure that the added gene won't be expressed.
Ie, if i knock out E-cadherin (targeting the first exon), and then add an apoptotic gene under the control of part of the E-cadherin upstream promoter sequence, will that apoptotic gene be expressed or not?
Legitemedly, sure it would not be expressed, because as you knock-out the first exon probably by frameshift, all you get is residual of proteins which are not functional and decays swift. Sometimes, however, there are secondary starting codons in different part of exons which can produce sort of pseudogenes but rearly functional.
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