Hi there! I am working with dual targeting CAR-T design. I used two strategies to achieve dual CAR expression in one cell: 2A and independent promoters. Unfortunately until now, neither of the above methods works for me! Only one of the two genes can be expressed. Can anyone give some specific advice? Thank you very much!
Dear Wenzhong...
To overcome the limitation, you may design a CAR that uses an antibody in its natural configuration for binding. Such CAR consists of two chains, the immunoglobulin light and heavy chain with their constant regions, whereby the heavy chain is anchored to the membrane and linked to an intracellular signalling domain for T-cell activation. The two chains form a stable heterodimer, a so-called dual chain CAR (dcCAR), and bind with high affinity and in a specific manner to their cognate antigen.
Reference:
https://www.nature.com/articles/gt201648
I hope this may help!
Regards.
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