Dose novobiocin have UV absorbance? and How can I draw standard curve of it? I mean which concentrations , do I need for standard curve??

02 January 2024 1 6K Report

I wanted to draw standard curve of Novobiocin in phosphate buffer(7.4), and I made 2,4,8,16,32 and 40 microgram/ml. but It did not show any absorbance in UV spectrometer. I want to know its problem.

Robert Adolf Brinzer

Even around 390nm?

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