To check for microbial contamination, there are devices that determine the amount of ATP by colorimetric method. Is the data obtained from this device acceptable in scientific articles? Is the use of ATP, in principle, to be an appropriate indicator for determining the level of contamination with bacteria?
ATP measurements are usually used for monitoring microbial drinking water quality. Many studies have also revealed that intracellular ATP levels differ between microbial taxa ( bacteria, fungi and other microbes as well as food and food debris, including that on disinfected surfaces. The differences between bacterial and aesthetic cleanliness lie at the the opposite ends of the spectrum in terms of relevance to diseases pertinent to food safety. Because the level is strictly - controlled in a living cell, ATP determination is used as an indicator of viable organisms. For more details consult orbit.dtu.dk --datastreams ; https://www.foodsafetymagazine.com
Dear Mohammad,
Measuring microbial ATP has been considered to be superior to the culture based methods due to the rapid availability of results and the fact that all microbes present in a sample both culturable and non-culturable cells. But this method non-specific and not as sensitive as the culture based methods for specific indicators and also results cannot be converted to a cell count.
Yohan
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